| Author | Comment | Last activity | Moderation | ||
|---|---|---|---|---|---|
| Ag8n | "It looks like a single beam instrument was used for this work. With a double beam instrument, there is no need for phrases like "OD435/OD415". Th..." | Read more » | over 2 years ago | |||
| Ag8n | " Lichen1.docx Hopefully, this will attach. " | Read more » | over 2 years ago | |||
| Ag8n | "In this area, lichens aren't common. Attached is a rough draft using lawn clippings to set up and test using various instruments. Don't know how ..." | Read more » | over 2 years ago | |||
| Ag8n | "The tungsten lamp remarks only apply to the 660 nm red lines. " | Read more » | over 2 years ago | |||
| Ag8n | "Best bet- use an old fashioned tungsten lamp. Not a LED claiming to be a tungsten lamp. An LED has a narrow bandwidth. An old fashioned tungsten..." | Read more » | over 2 years ago | |||
| Ag8n | "Looking into the 660 nm lines. It's not that LEDs don't exist for 425 nm region. They do. But the ones in the catalogs seemed expensive. Going ..." | Read more » | over 2 years ago | |||
| Ag8n | "There are 3 in 1 flashlights ( blue, white, and red) that would be good for this application- as long as the color was more like purple instead of ..." | Read more » | over 2 years ago | |||
| Ag8n | "If you are using the public lab spectrometer, a light source for around 400 nm is needed. It looks like there are some blue or violet led bulbs/ f..." | Read more » | over 2 years ago | |||
| Ag8n | "A test of this method might be to use lawn clippings and run it through the procedure. The idea is to extract chlorophyll, which should be presen..." | Read more » | over 2 years ago | |||
| Ag8n | "Another, more current option , is to use a coffee grinder. With the temperature issues this material shows, probably using the frozen state would ..." | Read more » | over 2 years ago | |||
| Ag8n | "As for the mortar and pestle, there are many options. Some mortar and pestles are very expensive, but they don't have to be. They should be easy t..." | Read more » | over 2 years ago | |||
| Ag8n | "Was checking one of the online vendors. Found new centrifuges for $70. Industrial quality centrifuge costs were much higher, but who knows. If y..." | Read more » | over 2 years ago | |||
| Ag8n | "Ok. Trying to stay as close to duplicate the original paper as closely as possible. R.la cera isn't in this area. But other closely related liche..." | Read more » | over 2 years ago | |||
| Ag8n | "One word of warning. Solvents ( like acetone) and plastic cuvettes do not usually go together. The worst case is the plastic melts, but the plast..." | Read more » | over 2 years ago | |||
| Ag8n | "Still have to do more work. But the acetone extract seems like something that might be doable in a home lab type situation. The acetone extract i..." | Read more » | over 2 years ago | |||
| fongvania | "There's some work needed to modify this research protocol so it can be done in regions like Ohio that might not have the exact species listed in th..." | Read more » | over 2 years ago | |||
| fongvania | "I don't know of a community science project using the pigment extraction method since there are some specialized steps and equipment, but we're cur..." | Read more » | over 2 years ago | |||
| Ag8n | "I went to ohiomosslichen.org/ to see what's in the ohio area. Didn't seem like there was anything directly comparable to the lichens in the paper..." | Read more » | over 2 years ago | |||
| Ag8n | "Out of curiosity only. " | Read more » | over 2 years ago | |||
| Ag8n | "Ok, the parameters are listed. Is there an example of a study set up? " | Read more » | over 2 years ago | |||
| Ag8n | "Thank you. That's what I was after. Much appreciated!!! " | Read more » | over 2 years ago | |||
| fongvania | "I have access to the paper you're talking about--here is a snippet of the section you may be most interested in: Let me check if I'm allowed t..." | Read more » | over 2 years ago | |||
| Ag8n | "Working my way through the paper. One of the things the original talks about is " Extraction by immersion in DMSO" and "OD435/OD415 nm". This is ..." | Read more » | over 2 years ago | |||
| fongvania | "Thank you for reading! Let me know if there's anything I can help clarify " | Read more » | over 2 years ago |