I am trying to determine the average amount of micro plastics in squid guts sourced from a local ...
Public Lab is an open community which collaboratively develops accessible, open source, Do-It-Yourself technologies for investigating local environmental health and justice issues.
This is a testing site only. See the live Public Lab site here »
All topics »
If you cannot use the ReCaptcha to verify you are not a bot, use this alternative verification.
As an open source community, we believe in open licensing of content so that other members of the community can leverage your work legally -- with attribution, of course. By joining the Public Lab site, you agree to release the content you post here under a Creative Commons Attribution Sharealike license, and the hardware designs you post under the CERN Open Hardware License 1.1 (full text). This has the added benefit that others must share their improvements in turn with you.
sign up to join the Public Lab community
Forgot your password? Reset it here
by plasticsquid |
February 20, 2020 16:31 |
I am trying to determine the average amount of micro plastics in squid guts sourced from a local grocery store. Does anyone know how many squids I should dissect to find this out?
This paper has some solid evaluation of the different ways to digest the squid guts: https://www.sciencedirect.com/science/article/pii/S0025326X19301845
This paper looked at 64 individual anchovies from local waters: https://www.researchgate.net/publication/308813073_Microplastic_fragments_and_microbeads_in_digestive_tracts_of_planktivorous_fish_from_urban_coastal_waters
I think that you can pick any number of fish to fit the amount of time and materials you can allocate to the project. If you would like to choose a number that has statistical significance, this thread has some information and resources on how to select the sample size for an experiment involving animal subjects that you could adapt: https://www.researchgate.net/post/How_can_I_calculate_the_sample_size_in_an_animal_trial
Reply to this comment...
Log in to comment
@mimiss has marked @amallozzi as a co-author.
Here’s a link to the chapter on determining sample size in an ecological study from Charles Krebs’ Ecological Methodologies textbook: https://www.zoology.ubc.ca/~krebs/downloads/krebs_chapter_07_2017.pdf
He walks the reader through a couple of standard options for how to figure this out based on the requirements you have for your results. He also provides an option near the end of the chapter for if none of the standard ways work for your study.
There are also sample size calculators available on the internet, but I think the problem you might run into with those is knowing how big your population size should be.
This is part of: