| Author | Comment | Last activity | Moderation | ||
|---|---|---|---|---|---|
| dcanprdn | "Absorbance is a chemical definition of how well a dissolved substance attenuates light at a particular wavelength as light passes through a solutio..." | Read more » | almost 7 years ago | |||
| cfastie | "I am not familiar with the optics equations. My approach would be to enable precise adjustments of the components and find the best resolution (cri..." | Read more » | over 7 years ago | |||
| gpotters | "@cfastie: well, I recall that we made whole leaf spectra in the lab (me = biologist, Ph D of UAntwerp). Guess with a decent light source you can do..." | Read more » | almost 9 years ago | |||
| vanjo63 | "I am going to try the experiment with an extraction of grass pigments by ethanol even if I don't think there is a big difference. That will allow t..." | Read more » | almost 9 years ago | |||
| cfastie | "I have wondered about how important it is to really extract the plant pigments for this type of absorbance observation. If you had an intact transp..." | Read more » | almost 9 years ago | |||
| gpotters | "Maybe you should try to do your extraction with some alcohol. Chlorophylls are, after all, lipophilic and you have to get them out of the chloropla..." | Read more » | almost 9 years ago | |||
| cfastie | "I guess you are right about the two peaks. I was thinking that you had captured the lesser peak of Chlorophyll a shown here at about 410 nm: Tha..." | Read more » | almost 9 years ago | |||
| dhaffnersr | "This is very very cool! I'm looking forward to more of your work. " | Read more » | almost 9 years ago | |||
| vanjo63 | "Oh tank you for this information, I didn't know that was possible to do that via the application Spectral Workbench 2 :) " | Read more » | almost 9 years ago | |||
| warren | "This is very cool, thanks for posting! I also wanted to point out that comparisons of this type are possible in Spectral Workbench 2: And so ar..." | Read more » | almost 9 years ago | |||
| vanjo63 | "In fact, chlorophyll a has 2 peaks : the first around 430 nm and the second around 660 nm ; while chlorophyll b has also 2 peaks : the first around..." | Read more » | almost 9 years ago | |||
| cfastie | "That's a good idea to use only one color of light. It can make it easier to interpret the result. Your result is very consistent with the two peaks..." | Read more » | almost 9 years ago |