Public Lab Research note


Oil fluorescence scanning on an Ocean Optics spectrometer

by warren | September 23, 2014 17:42 | 3,531 views | 2 comments | #11176 | 3,531 views | 2 comments | #11176 23 Sep 17:42

Read more: stable.publiclab.org/n/11176


What I want to do

At LEAFFEST 2014, Mary, Luisa, and others reproduced the kind of fluorescence spectrometry scanning we've been doing with the Oil Testing Kit using a lab spectrometer, an Ocean Optics device. We used the same 405nm laser pointer and the same samples -- of suspected BP crude, fish oil, and mail-ordered crude from ONTA, all dissolved in mineral oil, and plain mineral oil. Here are the graph and the files, which Louisa emailed to me:

oilsamples_oceanoptics.png

Update: Mary sent me specs on the device, below:

Spectra were measured with an OceanOptics SD2000 dual spectrometer (http://oceanoptics.com/wp-content/uploads/OEM-Data-Sheet-S2000.pdf), using the first spectrometer (wavelength range: 200-850nm; grating: 600 lines at 300nm; 25um slit).

Spectrometer is spectrally calibrated with a mercury/argon calibration source (http://oceanoptics.com/product/hg-1/). Values are relative DN, and are not radiometrically calibrated.


I had to add ".txt" to the end of each file to get them to upload, but that also makes them easier to open up.

BP_2014_256_15_59_12.fos.rdb.txt

Mineral_2014_256_16_01_28.fos.rdb.txt

Fish_2014_256_16_00_46.fos.rdb.txt

ONTA_2014_256_16_02_03.fos.rdb.txt

Questions and next steps

Presumably the calibration is OK? And the intensities are adjusted -- linear -- unlike our own spectrometer. So it's could be a basis for calibrating our device, if we assume that the setup is similar enough.

I'm especially interested in importing this data into Spectral Workbench, and am working on a way to upload this format. For now, you can view one of the files on Github too; they're just a column of wavelengths, a column of intensities, and an index column counting from 0 upwards:


2 Comments

Hi, check this out -- @hynae !

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"Kids, don't try this at home" (without eye protection ...)

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