Public Lab Research note


Expression of Violacein in Yeast?

by sara | March 15, 2012 19:17 | 3,717 views | 5 comments | #1416 | 3,717 views | 5 comments | #1416 15 Mar 19:17

Image from figure 2 of Martinelli et al. BMC Microbiology 2004 4:25 doi:10.1186/1471-2180-4-25

Following up on Avery's blog and research note, on present issues with the YES assay and BLYES assay for easy use outside of a lab. We are investigating whether estrogen responsiveness might be linked to the expression of purple pigment know as Violacein.

Violacein is pigment that has been extracted from amazonian bacteria see http://www.horizonpress.com/jmmb/v2/v2n4/26.pdf. It has been optimized to be used a reporter element by synthetic biologists: http://partsregistry.org/wiki/index.php/Part:BBa_K274002 and http://partsregistry.org/Part:BBa_K274002:Experience

We are interested to see if the yeast might be able to produce this pigment as well. The benefits of this using this pigment would be an assay for estrogens that is easily legible because the color of the yeast would change.

Some important questions to investigate are: 1. Are there potential hazards associated with violacein? Pigments like other chemicals can be biologically active. It will be interesting to investigate the other biological activities associated with this pigment. A good place to begin this research is: http://www.wikigenes.org/e/chem/e/5359453.html 2. Can yeast express Violacein? Synthetic biology teams are investigating whether the pigment can be expressed in yeast. We are presently following up with them: http://2011.igem.org/Team:Johns_Hopkins/Project/Violacein


5 Comments

I'm just curious, why violacein instead of any other reporters optimized for yeast expression?

I've been using violacein biobrick as part of high school student synthetic biology curriculum, and it actually covers the weird kinks (slow growth, unexpected behavior and etc etc) you need to be prepared for when working with such a huge (7~kb) part, that's actually an operon, even among similar chassis selection (like different strains of e.coli).

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Hi Sung, we're really just hoping for a reporter that would give an easily identifiable result. Are their other reporters you would suggest? We haven't started lab work on this yet so it would be great to hear ideas!

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Hi, this site might help for the expression but I'm NOT sure. http://www.creativebiomart.net/yeast-expression-systems.htm

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I can't open one of the link: http://www.wikigenes.org/e/chem/e/5359453.html Is this a site about gene? cdgenomics:http://www.cd-genomics.com

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Thanks for sharing these resource.

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