A refractometer measures how much light bends as it passes through a liquid. n = velocity of light in a vacuum / velocity of light in medium
Can a refractometer be used to check nutrient density in plant sap?
From the Real Food Campaign Website: Pure water at a specific temperature will bend light at a specific angle. A wide variety of biological substances can increase solution density, and thus cause light to refract (bend) more as it passes through a watery medium: 1. dissolved minerals (salts, acids, alkali) 2. carbohydrates (simple sugars) 3. amino acids (proteins) 4. lipids (special case: oil & water don't mix; form thin films) 5. almost any molecule with covalent bonding 6. very large suspended particles (colloids)
BRIX measures the percent solids (TSS) in a given weight of plant juice‚ nothing more‚ nothing less. BRIX is often expressed as the percentage of sucrose. However, the "sucrose" can vary widely. BRIX is actually a sum of the pounds of sucrose, fructose, vitamins, minerals, amino acids, proteins, hormones, and other solids in one hundred pounds of plant juice.
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I'd hazard a guess that a spectrometer would yield much more information about the contents of sap -- there's only one dimension in BRIX data, just one number.
Like, does higher BRIX mean more sugars, or more minerals, or what? There doesn't seem to be any way to tell; and with grapes, the original use case, Mathew points out that sugar is the key factor for fermentation. So is BRIX just showing higher sugar? Maybe that's valid since many fruits are "better" if they are sweeter.
What are the specific compounds nutrient-rich growers are trying to maximize? We can ask Severine a bit on Thursday, but it seems not unlikely that you'd be able to use a spectrometer to identify a spectral curve for what people call "good" vegetation and use that to assess other examples of that particular species of fruit/veg. Or if you're more interested in specific nutrients, we could compare the spectra to some way of measuring those to see if there's a strong correlation.
I found this nice outline of how a refractometer is used by one organic farmer on this discussion forum:
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Jeff - right, higher BRIX largely means higher sugars. It's interesting to consider building a reference library of spectra from "nutrient-dense" examples of particular crops to get measurements of more than suspended solids (largely sucrose). Or, alternatively, to start looking for specific minerals across multiple crops with a spectrometer.
I think the BRIX fans are imagining a day when there would be a simple-to-use consumer-level refractometer that someone would bring with them while grocery shopping.
By the way, it was very interesting to hear my farmer friend Deb Taft say that she was interested to use a refractometer in the field in order to train her eyes to recognize the signs of sucrose-dense crops. In other words, using a technological sensor to calibrate her own senses.
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I will tend to preface most of my participation on these forums with the caveat that I am far from an expert at most things, and will aim to bring more of the "citizen" perspective to PLOTS's science.
Lately, one of my mantras has been: when faced with a choice between two options - see if you can take both. As Jeff points out, BRIX measurements do not provide much information. Spectroscopy will provide a much richer portrait of any given sample. But "citizens" could not ask for a lower barrier to entry. E.g., take two drops of your tomato's juice, place it in the refractometer, and read the number. Higher is better, and here's a comparison chart for tomatoes generally, or for tomatoes in your region, or for tomatoes from this grower over the past several years.
That said, it's unclear to me where we could expect to see variation in the data, especially for fruits and vegetables that have been picked and transported. However, with enough data, you might begin to see interesting trends to bolster arguments that local produce is qualitatively better. But then there's that whole term "quality" - which is where I would hope more engaged citizen scientists equipped with spectrometers could provide some insight into the actual composition of the plant juices under test.
If the refractometer is cheap, simple to use, and easy to report with - I think this is a good introduction for citizen food science noobs.
gf
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I'd love to see a series of spectrometer measurements of a tomato as it ages, as it's kept in the fridge, or frozen.
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