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400nm LED and 405nm laser using a quartz cuvette

by dhaffnersr | March 05, 2016 18:49 05 Mar 18:49 | #12777 | #12777

I just got my quartz cuvettes in and I was very excited to use them and here are some results which I do have to say, make a huge difference in quality of spectral imaging and data collection:

Slit width used is .09mm

001ref_and_sample_uv400nm_LED_thru_quartz_cuvette_mar4.png

Reference used was 3ml of Ethanol in quartz cuvette for both the 400nm plots and the 405nm plots- sample was 0.001mol/l Rhodamine B (100ppm)(NIST standard prep is 100ppb) dissolved in Ethanol - 3ml sample vol.

zoomed_view001_ref_and_sample_uv400nm_LED_thru_quartz_cuvette_mar4.png

subtraction_of_ref_from_sample_uv400nm_LED_thru_quartz_cuvette_mar4.png

zomm_ed_view_subtraction_of_ref_from_sample_uv400nm_LED_thru_quartz_cuvette_mar4.png

Here are the results for the 405nm laser pointer scans:

ref_and_sample_uv405nm_thru_quartz_cuvette_mar4.png

zoom_view_ref_and_sample_uv405nm_thru_quartz_cuvette_mar4.png

subtraction_ref_from_sample_405nm_mar4_pic2.png

zoomed_view_subtraction_ref_from_sample_405nm_mar4_pic3.png

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