My first use of image-sequencer https://publiclab.github.io/image-sequencer/examples/#steps= so I am not sure what to expect..
Image examples are microscopic images of plant cells. IR image was collected with a 850nm LED. Color image with a white light LED. I know NDVI is designed for remote sensing applications so I am not sure if this is worthwhile? Also not sure if microscope slide and cover glass changes image spectral properties?
First image is a Pine Leaf example. Is that the right image sequence for NDVI analysis?
Second image is of a leaf and has a flat field image problem. Can you add flat field correction to Image sequencer? I believe the supplied version of the Raspberry Pi camera has it’s own flat field correction and we need to provide an alternate correction when the Pi camera is used with a different (objective) lens.
Image sequencer works great (amazingly easy to use) but I am a little nervous as to it how it actually works. My experience with photoshop and opencv is that processing steps often depend on many decisions that depend on the situation.