Tonight and tomorrow (October 8th) the Portland Science Hack Day will be running, and I’ll be joining Jenny Malloy with my OpenFlexure microscope to work on image analysis for sizing particles with ImageJ. In this note, I’ll provide some background, purpose and activities for getting started at analysing particle size under a microscope.
Sizing particles is the first step towards measuring airborne particles optically to bring accountability to the emissions of sand miners and other industrial operations. Read more on background research with the tag #passive-pm. Here is an example of a research study using passive particle monitors and visible light microscopy of particles.
Goals & Resources
My primary goal is for Image J’s measurement of particle diameter to correspond with the known diameter of standardized spheres (2.07 um polystyrene latex spheres).
My secondary goal is to figure out whether microscope calibration requires standardized spheres or if it can be done with a stage micrometer— a small ruler visible under a microscope.
Here are a series of scripts to try out:
Two more macros created with ImageJ's macro recording functions:
for use in calibrating a microscope.